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Peptide Storage Handling Guideline

Most lyophilized peptides shall be stable at room temperature for 1-2 weeks. For long term storage, it is strongly recommended that you store peptide in powder form at -20°C, away from strong light, and under dry condition. Repeated freeze-thaw cycles should be avoided. A peptide solution once prepared should be used as soon as possible. If storage in solution is unavoidable, use sterile buffers at pH 4-6 and store in aliquots at -20°C or lower to prolong the storage life. 

The shelf life of peptide solutions is limited, especially for peptides containing cysteine(C), methionine(M), tryptophan(W), asparginine(N), glutamine(Q), or N-terminal glutamic acid(E). For example, a Cys-containing peptide is easily oxidised, especially in basic conditions; some residues are easy to racemise, such as Proline. Avoid DMSO if the peptide contains Met, Cys or Trp, due to sulfoxide or disulfide formation. Peptide stability becomes worse when in a solution, especially at the higher pH (pH>8). We therefore recommend keeping solutions in the range of pH 4-6. It is recommended that peptides containing methionine, cysteine, or tryptophan residues be stored in oxygen-free atmosphere to avoid oxidation. The presence of dithiothreitol (DTT) can be useful in preventing oxidation. 

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